Question

Choose the correct with respect to RNA molecules.

(a) They are less labile than DNA molecules

(b) The 2’ hydroxyl group of ribose group decreases the activity

(c) There is no less of activity while boiling

(d) Baking of glassware, treatment with UV can be used for protection against degradation

This question was addressed to me during an online interview.

My question is taken from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis in division Libraries Construction of Genetic Engineering

Answer 1

The correct option is (d) Baking of glassware, treatment with UV can be used for protection against degradation

To elaborate: RNA molecules are more labile as compared to the DNA molecules and they are more reactive because of the 2’ hydroxyl group of ribose group. Baking of glassware, treatment with UV can be used as methods for protection against degradation. If precautions are not taken, there is subsequent loss of activity while boiling.