Question

The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

(a) True

(b) False

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This is a very interesting question from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in section Libraries Construction of Genetic Engineering

Answer 1

Correct answer is (a) True

The best explanation: A chemically synthesized oligo-dT primer is annealed to the 3’ polyA tail of RNA. After this primer is attached the RNA DNA duplex is formed with the help of reverse transcriptase.