Login

Remember

Register

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Ask a Question

Home
Genetic Engineering
What is done after RNA fractionation is carried...

What is done after RNA fractionation is carried out?

← Prev Question Next Question →

+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

What is done after RNA fractionation is carried out?

(a) Each band is translated in vivo

(b) Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

(c) Translation is carried out with a high background

(d) Amino acid is not radioactively labelled

The question was posed to me in an online interview.

The query is from Cloning the cDNA and Specialized Libraries in division Libraries Construction of Genetic Engineering

genetic engineering
libraries construction
cloning the cdna and specialized libraries

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

Correct answer is (b) Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

The best I can explain: RNA fractionation is carried out and it is followed by a translation of each band in vitro. It is carried out in wheat gram or lysate of rabbit reticuloycte cells. Ribosomes, tRNAs are added in order to carry out the translation with low background. An amino acid is radioactively labelled and thus the polypeptide sequence synthesized is labelled.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

Choose the correct statement for RNA fractionation.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the correct statement if the RNA is non polydenylated.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Any cDNA library would represent a fraction of RNA species of an organism.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

What do we mean by housekeeping genes?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

If physical methods are used, tracer is amplified and is labelled with biotin.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

The nucleic acid from the cell type that contains the sequence we are interested in is called ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Which of the following is desirable?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the correct statement in order to enrich rare species.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

For cloning purposes, the intact chromosomes should be separated by ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Which of the statement holds true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the correct statement for construction of a library subsection.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Which statement holds true for hydroxyapatite?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the incorrect statement for the homopolymer tailing of cDNA strands.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

Choose the correct statement for modification of homopolymer tailing method.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cloning the cdna and specialized libraries

+1 vote

1 answer

A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cdna libraries
polyadenylated rna and cdna synthesis

+1 vote

1 answer

What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cdna libraries
polyadenylated rna and cdna synthesis

+1 vote

1 answer

Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cdna libraries
polyadenylated rna and cdna synthesis

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

What is done after RNA fractionation is carried out?

Please log in or register to answer this question.

1 Answer

Please log in or register to add a comment.

Related questions

Categories