Question

Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

(a) amplification

(b) normalization

(c) selection

(d) narrowing

I had been asked this question in examination.

Enquiry is from Cloning the cDNA and Specialized Libraries topic in portion Libraries Construction of Genetic Engineering

Answer 1

Correct option is (b) normalization

For explanation I would say: If the required mRNA is present in less number, its representation can be increased by a process called normalization. It reduces the number of times the library has to be screened for required cDNA.