Question

For cloning purposes, the intact chromosomes should be separated by ___________

(a) agarose gel electrophoresis

(b) fluorescence- activated sorter

(c) polyacrylamide gel electrophoresis

(d) chromatography

The question was posed to me in final exam.

My question comes from Cloning the cDNA and Specialized Libraries in chapter Libraries Construction of Genetic Engineering

Answer 1

Correct option is (b) fluorescence- activated sorter

Explanation: For cloning purposes, the intact chromosomes should be separated by using fluorescence-activated sorter. The amount of fluorescence obtained is based on the amount of dye and thus depends on the size of the chromosome.