Question

Choose the correct statement in order to enrich rare species.

(a) The most basic principle relies on the kinetics of hybridization

(b) A collection of single stranded molecules is allowed to reanneal under given conditions

(c) The strands which are less abundant are able to find their complement easily

(d) The left over molecules are more abundant in nature

I had been asked this question during an online interview.

This interesting question is from Cloning the cDNA and Specialized Libraries topic in portion Libraries Construction of Genetic Engineering

Answer 1

The correct option is (a) The most basic principle relies on the kinetics of hybridization

To explain I would say: In order to enrich the rare species, the basic principle relies on the kinetics of hybridisation. A collection of double stranded cDNA molecules is allowed to melt. As the strands melt away, they are then allowed to reanneal under suitable conditions. The more abundant the species is, the more easily they anneal. The rare species are obtained in single stranded form because it is difficult to reanneal them.