Question

Choose the correct statement for modification of homopolymer tailing method.

(a) It includes modification of primers

(b) Primers are varied by simply altering their size by randomly adding or removing bases

(c) The 5’ end of the first cDNA strand is tailed with C residues

(d) A single stranded oilgonucleotide is then used for second strand synthesis

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Answer 1

Correct answer is (a) It includes modification of primers

Easiest explanation: The modification of homopolymer tailing method includes modification of primers. Primers can be modified by the introduction of restriction sites in them. The 3’ end of the first cDNA strand is tailed with C residues. Another oligo-dG primer precedes the introduced restriction site and is contained with the double stranded oligonucleotide region. It is used for second strand synthesis.