Question

Physical separation is also used at times. Choose the correct statement with respect to this method.

(a) Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

(b) In sugar density gradient, the molecules are separated on the basis of density

(c) The central portion is recovered from the gel or the gradient

(d) Gel is more efficient than the sugar density gradient

I have been asked this question in unit test.

I'm obligated to ask this question of Cloning in Lambda in section Other Vector Systems for E of Genetic Engineering

Answer 1

The correct answer is (a) Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

Easy explanation: Physical separation is also used at times, it constitutes of using gel electrophoresis or by using centrifugation in the sugar density gradient. In the sugar density gradient, the separation is done on the basis of size. The arms are separated from the gel or gradient. A gradient is more efficient than gel, thus gradient is more preferred over the gel.