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What is the half life cycle for Taq polymerase?

(a) 40 minutes

(b) 80 minutes

(c) 10 minutes

(d) 50 minutes

The question was asked during an interview for a job.

This intriguing question comes from The Basic Technique topic in portion Polymerase Chain Reaction of Genetic Engineering

1 Answer

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Best answer
The correct answer is (a) 40 minutes

Best explanation: The half life cycle for Taq polymerase is 40 minutes at 94 degrees. Thus, at the end of the 30 cycles, a significant loss of activity takes place.

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