Question

Taq polymerase incorporates which residue at 3’ end?

(a) G

(b) T

(c) A

(d) C

This question was posed to me in an interview for job.

I want to ask this question from The Basic Technique topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct answer is (c) A

To elaborate: It incorporates A residue at the 3’ end. This overhang is often useful in carrying out the cloning of these PCR products.