Question

Both the primers, the start primer and the end primer should have a nearly same melting temperature.

(a) True

(b) False

I had been asked this question by my college professor while I was bunking the class.

I need to ask this question from Primers topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct choice is (a) True

Explanation: Both the primers should have a nearly same melting temperature. It is so because then they would nearly bind at the same time as the temperature is being lowered for their annealing.