Question

Which of the statements don’t hold true for the forensics and the amplification carried out?

(a) In the case of forensics, conventional methods such as southern blotting are used very effectively

(b) In cases of bone fragments which contain less than 300 nucleotides conventional methods can’t be applied as they involve southern blotting, restriction digestion etc

(c) The poor condition of DNA also makes the PCR amplification difficult

(d) Microsatellites composed of simply varying repeats of CA sequences are used

I had been asked this question in my homework.

My question is from Applications in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct option is (a) In the case of forensics, conventional methods such as southern blotting are used very effectively

The best explanation: In the case of forensics conventional methods are not very useful. If the bone fragments are having less than 300 nucleotides then the conventional methods can’t be applied easily.