Question

Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

(a) It can be analysed by PCR

(b) It destroys a restriction site

(c) The mutation is in alpha globulin gene

(d) The conventional approach took weeks for the whole analyses to be carried out

I have been asked this question during an internship interview.

Origin of the question is Applications topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right choice is (c) The mutation is in alpha globulin gene

The explanation: Sickle cell anaemia is a mutation in the beta globulin gene. It destroys a restriction site and the analyses can be carried out by PCR. PCR is very fast in comparison to conventional methods which took weeks to be completed. And the former method is completed in just one day.