Question

PCR products can be analysed in many ways. Which of the following is not possible?

(a) Use of restriction enzymes

(b) Determining whether a particular oliginucleotide probe hybridizes to a PCR product

(c) Electrophoresis

(d) Direct sequencing can’t be carried out

I got this question during a job interview.

My question is taken from Applications topic in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Right option is (d) Direct sequencing can’t be carried out

To explain: Restriction enzymes can be used and analyses can be done via destroying the restriction site. Checking for the hybridization of oligonucleotide probe with a PCR product. Electrophoresis can be used to check the mobility and compare it with a wild type molecule. Direct sequencing can also be carried out in order to analyse the PCR product.