Question

A reaction mixture for PCR consists of ____________

(a) heat unstable polymerase

(b) primers in a limited amount

(c) deoxynucleoside triphosphate (dNTPs)

(d) a region complementary to the sequence to be amplified

I had been asked this question during an interview for a job.

I would like to ask this question from The Basic Technique in portion Polymerase Chain Reaction of Genetic Engineering

Answer 1

The correct answer is (c) deoxynucleoside triphosphate (dNTPs)

The best I can explain: A reaction mixture for PCR consists of the region of the DNA sequence to be amplified, primers in large molar excess, heat stable polymerase and dNTPs. Heat stable polymerase which is used commonly is Taq polymerase.