Question

Choose the incorrect statement for prehybridization mixture.

(a) The function of it is to carry out specific binding

(b) It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm

(c) It is added in a small amount

(d) It is carried out before the hybridization is carried out

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This intriguing question comes from Database Screening and Screening by Nucleic Acid Hybridisation in portion Screening Libraries of Genetic Engineering

Answer 1

The correct option is (a) The function of it is to carry out specific binding

The explanation: Prehybridization mixture is meant for blocking sites which are meant for non-specific binding. It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm. They are added in a small amount and it is added before the hybridization is carried out. So that when hybridization is carried out, only specific binding takes place.