Question

Choose the incorrect statement for panning.

(a) It is a variant of ligand binding approach

(b) The ligand is immobilized on the solid support and the cells are passed over it

(c) If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand

(d) They are not useful for scanning libraries in cultured mammalian cells

This question was addressed to me by my school teacher while I was bunking the class.

The origin of the question is Screening by Expression In Vivo in section Screening Libraries of Genetic Engineering

Answer 1

Correct choice is (d) They are not useful for scanning libraries in cultured mammalian cells

To elaborate: Panning is a variant of ligand binding approach. In this, the ligand is immobilized on the solid support and the cells are passed over. If the ligand binding domain is exposed on the cells they would bind to the ligand and would be recovered afterward. They are useful in screening libraries in cultured mammalian cells because the cell wall is not there and thus access is easy.