Question

In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

(a) secondary, primary

(b) primary, secondary

(c) primary, tertiary

(d) secondary, tertiary

This question was addressed to me in an interview for job.

Question is taken from Screening by Expression In Vivo topic in division Screening Libraries of Genetic Engineering

Answer 1

Correct option is (b) primary, secondary

To explain: In the case of immunochemical screening, the position of the primary antibody is detected with a secondary antibody. Secondary antibody is common for all the primary antibodies and thus detection becomes easier.