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The process of baking or cross-linking of the membrane is carried out by ___________

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The process of baking or cross-linking of the membrane is carried out by ___________

(a) By heating at 80 degrees

(b) By irradiating with UV

(c) Both irradiation and heating method are used

(d) By addition of agarose

This question was posed to me in an interview.

My doubt stems from Database Screening and Screening by Nucleic Acid Hybridisation topic in chapter Screening Libraries of Genetic Engineering

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Correct option is (c) Both irradiation and heating method are used

For explanation: The cross-linking or baking of membrane is carried out by either heating it at 80 degrees or irradiating with UV. It is important to carry out this step because it binds the DNA irreversibly to the membrane.

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