Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
Protein-protein interactions such as in electron...

Protein-protein interactions such as in electron transport lead to activation of the reporter gene.

← Prev Question Next Question →

+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

Protein-protein interactions such as in electron transport lead to activation of the reporter gene.

(a) True

(b) False

The question was posed to me during an interview.

My question is from Reporter Genes in chapter Screening Libraries of Genetic Engineering

genetic engineering
screening libraries
reporter genes

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

Right choice is (b) False

Explanation: Protein-protein interactions are weak interactions and thus they are not sufficient for the activation of the reporter gene.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

_____ should enter the cell in the case of activation of the reporter gene for two hybrid system.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

Sequences that can function as origins of replication are called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

Promoter-probe vectors are used often. Choose the correct statement for these vectors.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

Luciferase genes are also used at times for detection. Choose the correct statement for them.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
screening by expression in vivo

+1 vote

1 answer

The ligand should bind to a single protein only for the screening process.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
screening by expression in vivo

+1 vote

1 answer

If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
screening by expression in vivo

+1 vote

1 answer

In the case of ligand binding by the expressed protein, the library can be screened by ______________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
screening by expression in vivo

+1 vote

1 answer

In ______ organisms, firstly the gene is looked into model organisms.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
expression of coding section in-vitro

+1 vote

1 answer

If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

If the library to be screened and the host belongs to different species, then it is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
screening by expression in vivo

+1 vote

1 answer

If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
expression of coding section in-vitro

+1 vote

1 answer

The process of finding a particular member of the library which is having some defined properties is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

The reporter gene in enhancer trap system is preceded by ______

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
gene function and gene trapping

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (481)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (404)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...