Question

What happens once the cosmid enters the E. coli cells?

(a) There is strict size selection inside E. coli cells

(b) Partial deletion may take place

(c) The tendency of deletion may is increased by using low copy number

(d) The tendency of deletion can’t be altered

The question was asked in an interview.

The above asked question is from Lambda ZAP and Cosmids in division Other Vector Systems for E of Genetic Engineering

Answer 1

The correct option is (b) Partial deletion may take place

Easy explanation: There is no size selection once the cosmid enters the E. coli cells. Because of it, partial deletion may take place. The tendency of deletion can be reduced by using a low copy number.