Question

Which of the following is not included as a step for cloning mini Mu in vivo?

(a) A culture of target strain, whose DNA we want to clone is infected with mini Mu

(b) The phage is inserted at multiple sites in the genome

(c) Some of the packaged strain is flanked by two mini Mu genes

(d) The packaged phage is introduced into a second strain and Mu would replicate as a phage

This question was posed to me in quiz.

Question is from Bacteriophage-Mu and Bacteriophage-Pi in chapter Other Vector Systems for E of Genetic Engineering

Answer 1

The correct choice is (d) The packaged phage is introduced into a second strain and Mu would replicate as a phage

For explanation I would say: For cloning in mini Mu, a culture of target strain whose DNA we want to clone is infected with mini Mu. The phage is inserted at multiple sites in the genome. Some of the packages strain is flanked by two mini Mu genes. The packaged phage is introduced into a second strain and the Mu won’t be able to replicate as a phage.