Question

Which of the following is a restriction for cloning in bacteriophage Mu?

(a) It is limited to handling only small number of phage particles

(b) It relies on whether the DNA is flanked by two mini Mu genes or not

(c) Recombination between the elements in the second strain is not a restriction

(d) Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not

I had been asked this question in my homework.

My question is based upon Bacteriophage-Mu and Bacteriophage-Pi topic in division Other Vector Systems for E of Genetic Engineering

Answer 1

The correct choice is (b) It relies on whether the DNA is flanked by two mini Mu genes or not

For explanation: The cloning in bacteriophage Mu is restricted by some factors. These include, it depends whether the DNA is flanked by two mini Mu genes or not. Recombination between the elements in the second strain is also important. Fragments of only those genomic DNA can be cloned, in which Mu can replicate.