Question

The presence of enzyme in the sample can be detected by _________________

(a) gel exclusion chromatography

(b) affinity chromatography

(c) ion exchange chromatography

(d) thin layer chromatography (TLC)

The question was asked in an interview for job.

My doubt is from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

Answer 1

The correct choice is (d) thin layer chromatography (TLC)

For explanation I would say: Enzyme which have been clarified and concentrated can be detected by thin layer chromatography. Gel exclusion, affinity and ion-exchange chromatography are used for purification of enzymes based on the property of the enzyme.