Question

Freezing technique is not preferred unless otherwise there is a need.

(a) True

(b) False

This question was addressed to me in a job interview.

My enquiry is from Extraction of Enzymes by Physical Methods topic in section Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

The correct answer is (a) True

To explain: Freezing involves the cells to be subjected to very cold minus degree Celsius temperatures which may lead to formation intra and extra cellular ice crystals damaging the cells. Also, there is chance of protein denaturation. Hence this technique is not preferred unless otherwise there is a need.