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A megaprimer method is a ____ stage approach and...

A megaprimer method is a stage approach and uses _ oligonucleotide primers.

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+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

(a) two, two

(b) two, three

(c) one, two

(d) one, three

This question was posed to me in an interview for job.

My question is taken from Mutagenesis using PCR and Recovery of Mutated Sequences in chapter Modifications and Mutagenesis of Genetic Engineering

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

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1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

The correct choice is (b) two, three

Easiest explanation: A megaprimer method is an approach of carrying out mutagenesis by using PCR. It is a two stage approach and uses three oligonucleotide primers.

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