Question

Choose the incorrect statement for the method based on antisense RNA.

(a) RNA is synthesized complementary to the sequence of the gene which is to be inactivated

(b) It is achieved by placing a DNA sequence which encodes RNA complementary to the RNA to be inactivated

(c) Expression of the endogenous gene is diminished

(d) This method is preferred over gene disruption has gene inactivation can be achieved completely

I got this question by my school teacher while I was bunking the class.

This is a very interesting question from Post-transcriptional Gene Silencing in division Modifications and Mutagenesis of Genetic Engineering

Answer 1

The correct answer is (d) This method is preferred over gene disruption has gene inactivation can be achieved completely

Easy explanation: Antisense RNA is the RNA which is complementary to the RNA sequence which is to be inactivated. It is done with the help of a DNA sequence. Expression of the endogenous gene is diminished. This method doesn’t lead to complete inactivation, which is not the case with gene disruption.