Question

Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.

(a) True

(b) False

This question was addressed to me in an online interview.

My doubt stems from Choosing the Right Mutations, Database of Mutant Lines and Gene Disruption topic in division Modifications and Mutagenesis of Genetic Engineering

Answer 1

Correct choice is (a) True

The best explanation: The incoming molecule having the marker will not replicate stably because it is linear. For the stable replication, double crossover of the flanking sequence should take place, replacing the endogenous gene with the disrupted gene.