Question

What is the basis of RNaseH method?

(a) It is based on RNA synthesis by DNA strand

(b) It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

(c) It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme

(d) It is based on getting double strand RNA from a single strand

I have been asked this question during an interview for a job.

My question is taken from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis topic in section Libraries Construction of Genetic Engineering

Answer 1

The correct option is (b) It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

Explanation: RNase H method is based on the synthesis of the complementary DNA synthesis by RNA strand through reverse transcriptase enzyme and it leads to the formation of the duplex of RNA and DNA.