Question

Choose the incorrect statement for oligo-dT cellulose.

(a) It is used for separation of polyadenylated mRNA from another mRNA

(b) oligo-dT are covalently attached to the solid support via OH bonds

(c) A solution containing RNA is passed through the column

(d) Poly A tail attaches to the oligo-dT by ionic bonds

The question was posed to me in an interview for job.

Question is from cDNA Libraries, Polyadenylated RNA and cDNA Synthesis in chapter Libraries Construction of Genetic Engineering

Answer 1

Right choice is (d) Poly A tail attaches to the oligo-dT by ionic bonds

The best I can explain: oligo-dT cellulose is used for separation of polyadeylated mRNA from another mRNA. These are covalently attached on a solid support via OH bonds. For separation, a solution containing RNA is passed through the column and the molecules having poly A tails are attached to the column via hydrogen bonds.