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in Enzyme Technology by (55.0k points)
Immobilization can greatly affect the stability of the enzyme.

(a) True

(b) False

This question was posed to me by my school principal while I was bunking the class.

My doubt stems from Kinetics of Immobilized Enzymes topic in section Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

1 Answer

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Best answer
Correct answer is (a) True

Best explanation: The above statement is true. Immobilization can greatly affect the stability of the enzyme due to the following reasons:

Immobilization protocol may produce some strain on the enzyme which may lead to inactivation of the enzymes under denaturing condition conditions of pH and temperature.

As there is a substantial multipoint binding between the enzyme and the support, there is good amount of stabilization.

Immobilization also protects the enzyme from microbial attack, thus enhancing stability.

Enzyme and support must be complimentary with the formation of unstrained covalent and non-covalent interactions.

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