Question

What is the correct statement with respect to ddNTPs?

(a) They are dideoxynucleotide triphosphates

(b) They are used in the termination of DNA sequencing

(c) They are used for initiating DNA sequencing

(d) They are used in the case if the starting amounts are large

The question was posed to me by my college director while I was bunking the class.

My question comes from Applications in chapter Polymerase Chain Reaction of Genetic Engineering

Answer 1

Correct option is (b) They are used in the termination of DNA sequencing

To explain I would say: ddNTPs are dideoxynucleoside triphosphates. It is used in the case for termination of sequencing. It is so because, both the hydroxyl molecules are removed sequencing would be terminated. It is beneficial in the case if the initial amounts are less.