Question

___________ are used to immobilize the enzyme to the aqueous phase allowing its separation from product-containing organic phase.

(a) Centrifuge

(b) Chromatography

(c) Phase separators

(d) Ultrasonicator

I have been asked this question in a national level competition.

This intriguing question originated from Recent Advances topic in chapter Recent Advances in Enzyme Technology of Enzyme Technology

Answer 1

Correct option is (c) Phase separators

To explain: Restriction of the enzyme to the aqueous phase effectively immobilizes the enzyme and allows its straightforward separation from product-containing organic phase by use of phase separators. Centrifuge is used for centrifugation which separates on the basis of the particle size and density difference between the liquid and solid phases. Chromatography is used for separation of a mixture by dissolving the mixture in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. Ultrasonicator is utilized to break up, disperse and sheer target material samples through its focused ultrasonic frequencies.