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Which of the following enzymes do not use Mg^2+ ions as cofactors?

(a) Hexokinase

(b) Pyruvate kinase

(c) Glutathione peroxidase

(d) Glucose-6-phosphatase

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The question is from Enzymes in portion Enzymes of Enzyme Technology

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The correct choice is (c) Glutathione peroxidase

The best explanation: Cofactors are additional non protein chemical components required for optimum activity of the enzyme. The enzymes which use Mg^2+ ions as cofactors are hexokinase, pyruvate kinase, and Glucose-6-phosphatase. For glutathione peroxidase enzyme, Se^+ is used as the cofactor.

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