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Which of the following represents a commonly used family of mediators?

(a) Pyruvate kinase

(b) Hexokinase

(c) Ferrocenes

(d) Lactate oxidase

I had been asked this question during an online exam.

Query is from Amperometric Biosensors topic in division Biosensors of Enzyme Technology

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Right option is (c) Ferrocenes

For explanation: Ferrocenes represent a commonly used family of mediators. An extreme case of this amplification is the detection of ADP. ADP is the added analyte and excess glucose, phosphoenol pyruvate, NADH and oxygen are present to ensure maximum reaction. Four enzymes such as hexokinase, pyruvate kinase, lactate dehydrogenase and lactate oxidase are co-immobilised within the packed bed reactor.

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