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What is represented in the following diagram?

(a) Optical biosensor

(b) Amperometric biosensor

(c) ELISA

(d) Immunosensor

I got this question by my school teacher while I was bunking the class.

My question comes from Biosensors in portion Biosensors of Enzyme Technology

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Correct option is (d) Immunosensor

Explanation: Principle of Immunosensor is represented in the diagram. ELISA is performed initially and then the following steps are followed:

• A transducer is coated with immobilized antibody. The transducer is immersed in a solution containing a mixture of a known amount of antigen-enzyme conjugate plus the unknown concentration of sample antigen.

• After a suitable period, the antigen and antigen-enzyme conjugate will be distributed between the bound and free states dependent upon their relative concentrations.

• Unbound material is washed off and discarded. The amount of antigen-enzyme conjugate bound is determined directly from the transduced signal.

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