+1 vote
in Enzyme Technology by (55.0k points)
Use of mediators in amperometric biosensor is required.

(a) True

(b) False

I had been asked this question in an internship interview.

My question comes from Amperometric Biosensors topic in portion Biosensors of Enzyme Technology

1 Answer

+2 votes
by (727k points)
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Best answer
The correct choice is (a) True

Easy explanation: In amperometric biosensor, there is an efficient reduction of oxygen at the surface of the cathode causes the oxygen concentration there to be effectively zero. But the major problem in this case is their dependence on the dissolved oxygen concentration. To overcome this, mediators may be used which transfers the electrons directly to the electrode bypassing the reduction of the oxygen co-substrate. Hence the above statement is true.

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