The correct option is (c) ELISA
For explanation: The diagram represents the principle of ELISA. Steps involved in ELISA are:
• Antibody is immobilised on the surface of a tube and a mixture of a known amount of antigen-enzyme conjugate plus unknown concentration of sample antigen is placed in the tube and allowed to equilibrate.
• After a suitable period, the antigen and antigen-enzyme conjugate will be distributed between the bound and free states dependent upon their relative concentrations.
• Unbound material is washed off and discarded. The amount of antigen-enzyme conjugate that is bound may be determined by the rate of the subsequent enzymic reaction.