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What is represented in the following diagram?

(a) Piezo-electric biosensor

(b) Calorimetric biosensor

(c) ELISA

(d) Immunosensor

I got this question by my college professor while I was bunking the class.

My question comes from Biosensors in division Biosensors of Enzyme Technology

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The correct option is (c) ELISA

For explanation: The diagram represents the principle of ELISA. Steps involved in ELISA are:

• Antibody is immobilised on the surface of a tube and a mixture of a known amount of antigen-enzyme conjugate plus unknown concentration of sample antigen is placed in the tube and allowed to equilibrate.

• After a suitable period, the antigen and antigen-enzyme conjugate will be distributed between the bound and free states dependent upon their relative concentrations.

• Unbound material is washed off and discarded. The amount of antigen-enzyme conjugate that is bound may be determined by the rate of the subsequent enzymic reaction.

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