Question

What is represented in the following diagram?

(a) Protein engineering cycle

(b) Site directed mutagenesis

(c) Polymerase chain reaction

(d) Transcription

This question was addressed to me at a job interview.

Question is from Future Prospects topic in portion Enzyme Technology Future Prospects of Enzyme Technology

Answer 1

The correct choice is (a) Protein engineering cycle

The best I can explain: The diagram represents a protein engineering cycle. The steps of the cycle are as follows:

• First step is isolation and characterization of the required enzyme.

• This follows the analysis of information gathered along with the database of known structural effects of amino acid substitutions to produce a possible improved structure.

• Then, the enzyme of interest is constructed by site-directed mutagenesis, isolated and characterized.

• After this, the results obtained, successful or unsuccessful, are added to the database, and the process repeated until the required result is obtained.