Question

Which of the following system is not used to derivatize coenzymes?

(a) Alkylation of the exocyclic N6-amino nitrogen of the adenine moiety present in the coenzymes

(b) High molecular weight water-soluble derivatives

(c) Derivative of myoglobin acts as an effective natural ascorbate oxidase

(d) Enzymic regeneration

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Answer 1

Right option is (c) Derivative of myoglobin acts as an effective natural ascorbate oxidase

Easiest explanation: Derivative of myoglobin acts as an effective natural ascorbate oxidase and is achieved by attaching (Ru(NH3)5)^3+ to three surface histidine residues. This is an example of production of synzyme. The following systems are used to derivatize coenzymes:

• Coenzyme derivatization involves the alkylation of the exocyclic N6-amino nitrogen of the adenine moiety present in the coenzymes NAD^+, NADP^+, NADH, NADPH, ATP and coenzyme A.

• High molecular weight water-soluble derivatives such as dextrans, polyethyleneimine and polyethylene glycols may be used are they cause less diffusional resistance than insoluble coenzyme matrices.

• Enzymic regeneration is advantageous because of its high specificity. Enzymic processes utilizes cheap substrates and readily available enzymes and give non-interfering and easily separated products. Formate dehydrogenase and acetate kinase are the useful examples in present time.