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The property of increasing the amount of the enzyme by increasing the number of gene copies is used increase the activity of __________ in E. Coli.

(a) Subtilisin

(b) Trypsin

(c) Penicillin-G-amidase

(d) Glucose isomerase

I have been asked this question during an online exam.

The question is from Future Prospects in portion Enzyme Technology Future Prospects of Enzyme Technology

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Correct answer is (c) Penicillin-G-amidase

Easy explanation: The property of increasing the amount of the enzyme by increasing the number of gene copies is used increase the activity of penicillin -G-amidase in E. coli.  Enzymes may be redesigned to fit more appropriately into industrial processes, as in case of glucose isomerase which is made less susceptible to inhibition by the Ca^2+ present in the starch saccharification processing stream. Subtilisin has been aimed at the improvement of its activity in detergents by stabilizing it at even higher temperatures, pH and oxidant strength. Trypsin has an active site closely related to that of subtilisin and hence genetic engineering in trypsin gives the predictable result of abolishing the activity against its normal substrates, but unpredictably gives no activity against substrates where these basic residues are replaced by aspartic acid or glutamic acid.

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