Question

Which of the following is not a strategy to produce synzymes?

(a) Monoclonal antibodies covalently attached to the phosphonate esters which can act as esterases

(b) Molecular imprinting of polymers

(c) Primary amines alkylated with 1-iodododecane and 4(5)-chloromethylimidazole

(d) Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans

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Question is taken from Future Prospects topic in portion Enzyme Technology Future Prospects of Enzyme Technology

Answer 1

Right option is (d) Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans

The explanation is: “Synthesis of cyclodextrins from starch by the cyclomaltodextrin glucanotransferase from Bacillus macerans” is not a strategy to produce synzyme. Following are the strategies to produce synzymes:

• Monoclonal antibodies that are raised to immunize protein conjugates covalently with phosphonate esters which helps them to act as esterases.

• Molecular imprinting of polymers with the help of transition state analogues to shape polymerizing resins or inactive non-enzymic protein during heat denaturation.

• Primary amines from polyethyleneimine are alkylated with 1-iodododecane and 4(5)-chloromethylimidazole to produce synzyme which has 27% of the activity of α-chymotrypsin against 4-nitrophenyl esters.