Question

Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included?

(a) The single stranded molecule entering the cell is single stranded and is called as a + strand

(b) The single stranded molecule is converted into double stranded molecule which is called as replicative form (RF)

(c) It is carried out in a process similar to normal replication process of E. coli

(d) The positive strand is used for the transcription of viral proteins

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The query is from BAC Vector, M13 and its Derivatives topic in section Other Vector Systems for E of Genetic Engineering

Answer 1

Right choice is (d) The positive strand is used for the transcription of viral proteins

Explanation: Once the bacteriophage enters the bacterial cells it is converted into double stranded form from the single stranded form. The double stranded form is known as replicative form (RF). The single strand enteri g is called as a + strand. It is carried out in a process similar to that the normal replication process. A specific origin of synthesis is used for transcription of the complementary negative strand. The minus strand can be used to synthesize viral proteins.