Question

Choose incorrect statement for the substitution vector EMBL4.

(a) It is having two single multiple cloning sites

(b) The region to be removed is on one side of the multiple cloning sites

(c) If a digestion within the multiple cloning sites, it generates central portion and right and left arms

(d) The right and left portions are called as arms

The question was asked in an online interview.

Enquiry is from Cloning in Lambda topic in division Other Vector Systems for E of Genetic Engineering

Answer 1

The correct answer is (b) The region to be removed is on one side of the multiple cloning sites

To explain: The substitution vector EMBL4 contains two multiple cloning sites. The two multiple cloning sites flank the region which is to be removed. If digestion is done within the multiple cloning sites, it generates central portion and the right and left portions. The right and left portions separated are known as arms.