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Which of the following method is not reliable for viability tests?

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+1 vote
in Fermentation Technology by (72.1k points)
Which of the following method is not reliable for viability tests?

(a) Plate counts

(b) Methylene blue/Safranin O double stain

(c) Alkaline methylene violet test

(d) Citrate methylene violet

This question was posed to me in a national level competition.

My enquiry is from Yeast Condition and Surplus Yeast in portion Yeast Management & Microbiology of Fermentation Technology

1 Answer

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Best answer
Correct answer is (b) Methylene blue/Safranin O double stain

Explanation: Methylene blue/Safranin O double stain failed to distinguish between viable and non-viable cells. Therefore, it is not a reliable method for accessing the yeast condition, particularly in the exponential phase of the growth.

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