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In which of the following method microorganisms do not grow on the surface of the media?

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in Fermentation Technology by (72.1k points)
In which of the following method microorganisms do not grow on the surface of the media?

(a) Spread plate

(b) Pour plate

(c) Membrane filtration

(d) Surface fermentation

I had been asked this question in an interview for job.

Origin of the question is Microbiology in section Yeast Management & Microbiology of Fermentation Technology

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Best answer
The correct option is (b) Pour plate

For explanation: In spread plate method and membrane filtration techniques the microorganisms grow on the surface of the medium. Whereas in the pour plate technique the sample is directly added to the hot medium and the organisms grow within the medium. Surface fermentation is the growing of organisms on the surface, hence the name.

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