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Which of the following is used in the Gram’s staining?

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in Fermentation Technology by (72.1k points)
Which of the following is used in the Gram’s staining?

(a) Methylene blue

(b) Safranin

(c) Phenolphthalein

(d) Methyl orange

The question was asked in my homework.

This intriguing question originated from Microbiology topic in portion Yeast Management & Microbiology of Fermentation Technology

1 Answer

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Best answer
Correct choice is (b) Safranin

Best explanation: Safranin is used in Gram’s staining procedure. If it is a Gram-negative bacteria, it stains red. If it is a Gram-positive bacteria, it stains purple. The safranin is a counterstain and is lighter than crystal violet. It does not disrupt the colouration in Gram-positive cells while it decolorizes Gram-negative cells.

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