Question

Immobilized enzymes are more preferred over free enzymes in producing biosensors.

(a) True

(b) False

The question was asked in an international level competition.

My question is taken from Biosensors in division Biosensors of Enzyme Technology

Answer 1

Right choice is (a) True

The explanation is: Immobilized enzymes are more preferred over free enzymes in producing biosensors because of the following reasons:

• Immobilized enzymes may be re-used ensuring the same catalytic activity for a series of analyses.

• Immobilized enzymes are intrinsically stabled during the immobilization process

• Even if inactivation of enzymes occurs, it is steady and predictable

• Immobilised enzyme systems are re-usable up to 10,000 times over a period of several months which results in a considerable saving in terms of cost of the enzyme in bound state relative to that of free soluble enzymes.