Question

For measuring using calorimetric biosensors, the sensitivity of the glucose analysis using glucose oxidase may be doubled by the co-immobilization of _____________

(a) penicillinase

(b) uricase

(c) catalase

(d) urease

I had been asked this question during a job interview.

This is a very interesting question from Calorimetric Biosensors in portion Biosensors of Enzyme Technology

Answer 1

Right answer is (c) catalase

For explanation: In the simplest case of increasing sensitivity, the process can be linked to other several reactions in a reaction pathway which can contribute to the heat output. Thus, the sensitivity of the glucose analysis using glucose oxidase can be more than doubled by the co-immobilization of catalase within the column reactor in order to disproportionate the hydrogen peroxide produced.