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An extreme case of amplifying sensitivity for the determination of ADP does not involve co-immobilization which of the following enzyme?

(a) Hexokinase

(b) Lactate dehydrogenase

(c) Pyruvate kinase

(d) Catalase

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Right option is (d) Catalase

The best explanation: Catalase is co-immobilized with glucose oxidase within the column reactor in order to disproportionate the hydrogen peroxide produced. This doubles the heat output which can then be measured. To measure ADP, four enzymes such as hexokinase, pyruvate kinase, lactate dehydrogenase and lactate oxidase are co-immobilised within the packed bed reactor. In spite of the positive enthalpy of the pyruvate kinase reaction, the overall process results in a 1000-fold increase in sensitivity, primarily due to the recycling between pyruvate and lactate.

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